Expression of Serratia marcescens extracellular proteins requires recA
نویسندگان
چکیده
منابع مشابه
An Extracellular Nuclease from Serratia marcescens
The specificity of an extracellular nuclease from Serraiia marcescens was examined with both RNA and DNA as substrates. The enzyme was found to act in a predominantly endonucleolytic manner with less than 2% of the degradation products being mononucleotides. Both DNA and RNA were degraded to di-, tri-, and tetranucleotides terminating in 5’-phosphoryl ends. The enzyme had the unusual property o...
متن کاملDifferential secretion of isoforms of Serratia marcescens extracellular nuclease.
Extracellular secretion of the Serratia marcescens nuclease occurs in a two-step process: (i) rapidly to the periplasm via a signal sequence-dependent pathway and then (ii) slowly to the extracellular growth medium without cell lysis. There are two major isoforms of the nuclease in the culture supernatant of S. marcescens. We have isolated, purified, and determined the sequences of both isoform...
متن کاملSerratia marcescens.
Over the last 30 years, Serratia marcescens has become an important cause of nosocomial infection. There have been many reports concerning the identification, antibiotic susceptibility, pathogenicity, epidemiological investigations and typing of this organism. Accurate identification is important in defining outbreaks. The API 20E system has been used widely, but is not individually satisfactor...
متن کاملSerratia marcescens
The nucleotide sequence of the proBA operon from a proline-hyperproducing mutant of Serratia marcescens was determined. Two base substitutions were found: one in theproBstructura1 gene, coding for y-glutamyl kinase (GK), and a second one in the promoter region of the operon. The former base substitution led to a change of the predicted amino acid at position 117 from an alanine to a valine in G...
متن کاملAn upstream sequence element required for NucC-dependent expression of the Serratia marcescens extracellular nuclease.
The Serratia marcescens extracellular nuclease gene, nucA, is positively regulated by the product of the nucC gene. In this study, the upstream region required for NucC-dependent nuclease expression was defined by using fusions to the gene encoding chloramphenicol acetyltransferase (cat). This sequence includes an element of hyphenated dyad symmetry identified previously as the binding site for...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1990
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.172.1.342-349.1990